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KMID : 0379119980260020287
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Korean Journal of Mycology
1998 Volume.26 No. 2 p.287 ~ p.292
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In vitro Formation of Cochliobolus nisikadoi , the Perfect State of Bipolaris coicis
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Abstract
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The perfect state of Bipolaris coicis, causing leaf blight of Job´s tears, was induced under in vitro conditions. Eighty nine isolates of the fungus were collected from 17 locations in Korea from 1994 to 1996. They were crossed on Sach´s nutrient agar, on which a piece of rice straw was placed, and incubated at 25℃. Pseudothecia were produced only by certain combinations of compatible isolates. Although pseudothecia were usually produced on rice straw two weeks after incubation, asci and ascospores were observed only in a few pseudothecia examined. The pseudothecia were black and globose with protruding ostiolar beaks. The locales were filled with a mass of hyaline and filamentous pseudoparaphyses. Asci were cylindrical to clavate and straight or slightly curved. The ascus wall was bitunicate with short stapes. Ascospores were filiform, hyaline, and arranged parallel to slightly coiled in the asci, measuring 143-166.4×2.6-3.8 ㎛. Perfect state of the fungus was identified as Cochliobolus nisikadoi (Tsuda, Ueyama & Nishihara Alcorn), based on the morphological characteristics.
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